RESEARCH INTERESTS

Regulation of microvessel permeability especially the roles of Ca2+, nitric oxide, cAMP and cGMP to modulate the permeability properties of venular microvessels; 2) Three dimensional modeling of the molecular and cellular structures which determine the permeability properties of microvessels (tight junctions; adherens junctions glycocalyx, and focal attachment); 3) Intracellular imaging techniques as a bridge between studies at the molecular and cellular level in endothelial cells and function of investigations of intact microvessels. Our laboratory uses microperfusion techniques to: a) measure the permeability properties on individual microvessels; b) load the cells forming the vessel wall with fluorescent indicators to measure intracellular composition; and c) to introduce transgenes into the cells of the vessel wall. Our goal is to understand the mechanisms whereby the endothelial cells and the spaces between the cells regulate water and solute fluxes between circulating blood and the body tissues.

He, P. and F.E. Curry (1991). Depolarization modulates endothelial cell calcium influx and microvessel permeability. Am. J. Physiol. 261:H1246-H1254.

F.E. Curry (1992). Modulation of venular microvessel permeability by calcium influx into endothelial cells. Invited review. FASEB J 6:2456-2466.

He, P. and F.E. Curry (1993). Albumin modulation of capillary permeability: the role of endothelial cell [Ca++]i. American Journal of Physiology 265 (Heart Circ. Physiol. 34):H74-H82.

He, P. and F.E. Curry (1993). Differential actions of cAMP on endothelial [Ca++]i and permeability in microvessels exposed to ATP. American Journal of Physiology 265:H1019-1023.

Curry, F.E (1994). Regulation of water and solute exchange in microvessel endothelium: studies in single capillaries. Landis Award Lecture. Microcirculation: 1:11-26.

He, P. and F.E. Curry (1994). Endothelial cell hyperpolarization increases [Ca++]i and venular permeability. Journal of Applied Physiology 76: 2288-2297.

Fu, B.M., F.E. Curry and S. Weinbaum (1995). A diffusion wake model for tracer ultrastructure-permeability studies in microvessels. Am. J. Physiol. 269:H2124-H2140.

He P. and F.E. Curry (1995). Measurement of membrane potential of endothelial cells in single perfused microvessels. Microvascular Research 50:183-198.

Weinbaum, S. and F.E. Curry (1995). Modeling the structural pathways for transcapillary exchange. Symposia of the Society for Experimental Biology, Symposium 49:323-345.

Bates, D.O. and F.E. Curry (1996). Vascular endothelial growth factor increases hydraulic conductivity of isolated perfused microvessels. Am. J. Physiol. 271:H2520-2528.

He, P., Zhang, X. and F.E. Curry (1996). Ca2++ entry through conductive pathway modulates receptor-mediated increase in microvessel permeability. Am. J. Physiol. 271:H2377-2387.

Fu, B., F.E. Curry, R.H. Adamson and S. Weinbaum (1997). A model for interpreting tracer labeling of interendothelial clefts. Ann. Biomedical Engineering 25:375-397.

He, P., B. Liu and F.E. Curry (1997). Effect of nitric oxide synthase inhibitors on endothelial [Ca2+]i and microvessel permeability. Am. J. Physiol. 272:H176-H185.

He, P., M. Zeng and F.E. Curry (1997). Effect of nitric oxide synthase inhibitors on basal microvessel permeability and endothelial cell [Ca2+]. Am. J. Physiol. 273: H747-H755.

Bates, D.O. and F.E. Curry (1997). Vascular endothelial growth factor increases microvascular permeability via a calcium dependent pathway. Am. J. Physiol. 273:H687-694.

Kajimura, M., M.E. OՄonnell and F.E. Curry (1997). Effect of cell shrinkage on permeability of cultured bovine aortic endothelia and frog mesenteric capillaries. J. Physiol. 503:413-425.

Fu, B., R.H. Adamson and F.E. Curry (1998). Test of a two pathway model for small solute exchange across capillary wall. Am. J. Physiol. 274:H2062-H2073.

Adamson, R.H., B. Liu, G. Nilson-Fry, L.L. Rubin and F.E. Curry (1998). Microvascular permeability and number of tight junctions are modulated by cAMP. Am. J. Physiol. 274:H1885-H1894.

He, P., M. Zeng and F.E. Curry (1998). Cyclic GMP modulates basal and activated microvessel permeability independently of [Ca2+]i. Am. J. Physiol. 274:H1865-H1874.

Curry, F.E. (1998). Regulation of capillary permeability in single perfused microvessels. In Connective Tissue Biology: Integration and reductionism (R. Reed and K. Rubin Eds) Portland Press London, pp 195-206.

Curry, F.E. (1998). Invited Editorial on "Gadolinium prevents high airway pressure-induced permeability increases in isolated rat lungs". J. Appl. Physiol. 1111-1112.

Michel, C.C. and F.E. Curry (1999). Microvascular Permeability. Physiological Review, 79:703-761.

Skinner, R.A., V.L. Tucker and F.E. Curry (1999). Acute Effects of Tumor Necrosis Factor on Conductivity of Mammalian Post Capillary. Journal of Trauma 47:486-491.

Kajimura, M. and F.E. Curry (1999). Endothelial cell shrinkage increases permeability through a Ca2+-dependent pathway in single frog mesenteric microvessels. J. Physiol. (London) 518:227-238.

Curry, F.E. and R.H. Adamson (1999). Transendothelial pathways in venular microvessels exposed to agents which increase permeability: The gaps in our knowledge. Editorial Comment. Microcirculation 6:3-5.

He, P. and F.E. Curry (1999). Use of confocal microscopy to measure [Ca2+]i in individual endothelial cells in single perfused microvessels. In "Microcirculation", International Academic Publisher, Beijing .